Interest in bioenergy in recent years has been stimulated by both energy security and climate change concerns. Fuels derived from agricultural crops offer the promise of reducing energy dependence for countries that have traditionally been dependent on imported energy. Nevertheless, it is evident that the potential for biomass production is heavily dependent on the availability of land and water resources. Furthermore, capacity expansion through land conversion is now known to incur a significant carbon debt that may offset any benefits in greenhouse gas reductions arising from the biofuel life cycle. Because of such constraints, there is increasing use of non-local biomass through regional trading. The main challenge in the analysis of such arrangements is that individual geographic regions have their own respective goals. This work presents a multi-region, fuzzy input-output optimization model that reflects production and consumption of bioenergy under land, water and carbon footprint constraints. To offset any local production deficits or surpluses, the model allows for trade to occur among different regions within a defined system; furthermore, importation of additional biofuel from external sources is also allowed. Two illustrative case studies are given to demonstrate the key features of the model. (C) 2011 Elsevier Ltd. All rights reserved.