A correct separation of solids from liquid fraction is crucial for a successful treatment of swine manure. For this reason an in-depth study of flocculant addition on different livestock wastewaters was carried out. Two flushed swine manure matrices, namely the mixture from nursery and feeder-to-finish pigs and the feeder-to-finish slurry alone, were tested for solids and nutrients removals from liquid fractions. The separation techniques applied were sieving and flocculation. A range of 80-200 ppm of polyacrylamide (PAM) followed by screening was employed in the case of flocculation treatment. The best results were observed when using the highest PAM dose in the matrix correspondent to the mixture of slurries. The removal rates in the liquid fraction were 73%, for total solids, 87% for volatile solids, 98%) for suspended total and volatile solids, 71% for chemical oxygen demand, 40% for total Kjeldahl nitrogen, and 34% for soluble phosphorus. Once the best PAM dose (120 ppm) was chosen, an anaerobic biodegradability study was performed in order to check the increase of methane production in the separated fractions by using the flocculant and the screen. The assay determined that the solid fractions biodegradability was constant at 79%. Meanwhile for the liquid fractions, an increase of 9% points was achieved with PAM-amendment when compared with 82% reached for the liquid fraction obtained by screening. (C) 2007 Elsevier Ltd. All rights reserved.