The objective of this study was to evaluate the dechlorination rate (from an initial concentration of 180 mu mol l(-1)) and synergistic effect of combining commercial Fe-0 and autotrophic hydrogen-bacteria in the presence of hydrogen, during TCE degradation process. In the batch test, the treatment using Fe-0 in the presence of hydrogen (Fe-0/H-2), showed more effective dechlorination and less iron consumption than Fe-0 utilized only (Fe-0/N-2), meaning that catalytic degradation had promoted transformation of TCE, and the iron was protected by cathodic hydrogen. The combined use of Fe-0 and autotrophic hydrogen-bacteria was found to be more effective than did the individual exercise even though the hydrogen was insufficient during the batch test. By the analysis of XRPD, the crystal of FeS transformed by sulfate reducing bacteria (SRB) was detected on the surface of iron after the combined treatment. The synergistic impact was caused by FeS precipitates, which enhanced TCE degradation through catalytic dechlorination. Additionally, the dechlorination rate coefficient of the combined method in MFSB was 3.2-fold higher than that of iron particles individual use. Results from batch and MFSB experiments revealed that, the proposed combined method has the potential to become a cost-effective remediation technology for chlorinated-solvent contaminated site. (c) 2008 Elsevier Ltd. All rights reserved.