In regions with a large wine production the usage of their natural waste to make biodiesel can result an interesting alternative. In this work, different methods of extraction, refining and transesterification of grape seed oil were assayed Two techniques of oil extraction were compared: solvent extraction and pressing. Two conventional transesterifications of the refined oil were carried out using methanol and bioethanol, being the methyl and ethyl ester contents higher than 97 wt%. Finally, several in situ transesterifications were done In situ transesterification did not reach either the oil yield extraction or the alkyl ester contents but the obtained biodiesel had better oxidation stability in comparison with the conventional process (C) 2010 Elsevier Ltd All rights reserved