Fractal kinetic analysis has been applied for the kinetics of enzymatic saccharification of cellulose. Based on the first order cellulose degradation kinetic, a fractal kinetic model with two parameters (rate coefficient and fractal exponent) has been developed. The model fits very well with the experimental data of enzymatic saccharification of cellulose under different conditions, including cellulase loading, nature of substrate, hydrolysis temperature, and addition of nonionic surfactant or polymer in the reaction medium. It indicates that the complex kinetics of enzymatic saccharification of cellulose can be described with the fractal kinetic model. The model exhibits that an addition of nonionic surfactant or polymer increases the rate coefficient and decreases the fractal exponent at the same time. A nonionic surfactant or polymer aqueous solution may be a potential medium engineering method for enzymatic saccharification of cellulose in biofuel industry. (c) 2010 Elsevier Ltd. All rights reserved.