The present work aims to use a two-stage biotrickling filters for simultaneous treatment of hydrogen sulphide (H(2)S), methyl mercaptan (MM), dimethyl sulphide (DMS) and dimethyl disulphide (DMDS). The first biofilter was inoculated with Acidithiobacillus thiooxidans (BAT) and the second one with Thiobacillus thioparus (BIT). For separate feeds of reduced sulphur compounds (RSC), the elimination capacity (EC) order was DMDS > DMS > MM. The EC values were 9.8 g(MM-S)/m(3)/h (BTU; 78% removal efficiency (RE); empty bed residence time (EBRT) 58 s), 36 g(DMDS-S)/m(3)/h (BIT; 94.4% RE; EBRT 76 s) and 57.5 g(H2S-S)/m(3)/h (BAT; 92% RE; EBRT 59 s). For the simultaneous removal of RSC in BIT, an increase in the H(2)S concentration from 23 to 293 ppmv (EBRT of 59 s) inhibited the RE of DMS (97-84% RE), DMDS (86-76% RE) and MM (83-67% RE). In the two-stage biofiltration, the RE did not decrease on increasing the H(2)S concentration from 75 to 432 ppmv. (C) 2010 Elsevier Ltd. All rights reserved.