This study was performed to investigate the effect of biogas-biodiesel fuel combustion on the emissions reduction and nanoparticle characteristics in a direct injection (DI) diesel engine. In order to apply the two biofuels, biogas was injected into a premixed chamber during the intake process by using two electronically controlled gas injectors, and biodiesel fuel was directly injected into combustion chamber by a high-pressure injection system. The in-cylinder pressure and rate of heat release (ROHR) were investigated under various fuel conditions for single-fuel (biodiesel) and dual-fuel (biogas-biodiesel) combustions. To evaluate the engine performances and exhaust emissions characteristics, the indicated mean effective pressure (IMEP) and exhaust emissions were also investigated under various test conditions. Furthermore, the particle number concentration and the size distribution of nanoparticles were analyzed by using a scanning mobility particle sizer (SMPS). In the case of dual-fuels, the peak combustion pressure and ROHR were gradually decreased with the increase of the biogas fraction in the dual-fuels. As the premixed ratios increased, ignition delay and combustion durations were prolonged compared to single-fuel mode. The dual-fuels combustion showed that the IMEP decreased slightly and maintained similar levels up to 20 degrees BTDC due to the retarded combustion phase. The concentrations of NO(x) emissions were decreased for all injection timings as the premixed ratio (r(p)) increased. The soot emissions in dual-fuel operations were significantly lower than those in the single-fuel mode (r(p) = 0), and decreased gradually as the premixed ratio increased, regardless of injection timing. A lower nanoparticle size distribution was observed at all premixed ratios for dual-fuel combustion compared to those of the single fuel mode. The number distribution of both nuclei and accumulation modes also decreased with an increase in the biogas fraction. A slight reduction in the total particle number and total volume for all premixed ratios was observed as the injection timing increased from TDC up to 20 degrees BTDC. (C) 2011 Elsevier Ltd. All rights reserved.