The purpose of this paper was to develop and validate a simple adapted method for the quality control of the determination of total esters, mono-, di-, and triacylglycerides and free and total glycerol content in methyl or ethyl biodiesel. Advantages of the method over the standard EN 14103 and EN 14105 methods include reduced sample preparation and analysis time and wide applicability to biodiesel containing naturally occurring heptadecanoic acids. The method correlates well with the standard EN 14103 and the EN 14105 methods. (C) 2011 Published by Elsevier Ltd.