| 초록 |
1,3-Diaminopropane (1,3-DAP) is a compound which can be used as an intermediate in the preparation of many applications, including plastics, sequestering agents, and herbicides. Herein, Escherichia coli is engineered for the production of 1,3-DAP through genome-scale in silico flux analysis to find the most efficient metabolic pathways. By comparing heterologous C4 and C5 pathways, the C4 pathway was found to be more efficient and Acinetobacter baumannii dat and ddc genes were introduced which encode 2-ketoglutarate 4-aminotransferase and L-2,4-diaminobutanolate decarboxylase respectively. The native ppc and aspC genes were overexpressed and the pfkA gene was deleted for increased productivity. Fed-batch fermentation of the final strain resulted in the production of 13 g l-1 of 1,3-DAP in 69 h with the productivity of 0.19 g l-1 h-1 in a glucose minimal medium. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557).] |
