| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
An engineering of β-lactamase zymogen to establish a protease assay method |
| 초록 |
We engineered an autoinhibited β-lactamase enzyme by fusion of a β-lactamase enzyme and its inhibitory protein (β-lactamase inhibitory protein, BLIP) via a peptide linker containing a protease cleavage site. The zymogen was optimized by engineering a circularly permutated β-lactamase, introducing mutations into BLIP for decreasing its binding affinity to β-lactamase, and changing the linker sequence. Using this zymogen, a sensitive protease assay method was developed in which activation of the zymogen by proteases produces an amplified absorbance signal. The approach reported here can be adapted for engineering of zymogens as biological sensors and components of synthetic signaling pathways. |
| 저자 |
최운종1, 김하진2, 윤현경1, 유태현2
|
| 소속 |
1Department of Molecular Science and Technology, 2Ajou Univ. |
| 키워드 |
β-Lactamase zymogen; Autoinhibition; MMP-2 activity assay
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| E-Mail |
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