| 학회 |
한국화학공학회 |
| 학술대회 |
2020년 가을 (10/14 ~ 10/16, e-컨퍼런스) |
| 권호 |
26권 1호, p.968 |
| 발표분야 |
기능성 결정화 분리공정 심포지엄(분리기술/열역학분자모사부문위원회) |
| 제목 |
One-step optical resolution of amino acid by preferential crystallization combined with enzymatic racemization |
| 초록 |
Many of the pharmaceutical compounds have asymmetric carbon. There is a need for techniques to reliably obtain one enantiomer. One of the methods of optical resolution is a preferential crystallization. In the preferential crystallization, crystals with high purity can be obtained by a simple operation. However, if chiral molecules do not racemize in solution, the maximum yield is 50%. By racemizing the chiral molecule in solution, other enantiomers can be converted to opposite enantiomer and crystallized as the desired product. So, the maximum yield would be 100%. In this study, the enzymatic reaction by racemase was employed for racemization. We aimed to improve yield by performing preferential crystallization of amino acids combining it with racemization by racemase. The racemase genes of original host bacteria were artificially synthesized and expressed in E. coli. The obtained racemase was used for cooling preferential crystallization of alanine and glutamic acid. By using enzymatic racemization, the undesired crystallization of opposite enantiomer was avoided, and the efficient chiral crystallization was realized. |
| 저자 |
Koichi Igarashi, Tomono Fujimura
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| 소속 |
Osaka City Univ. |
| 키워드 |
amino acid; chiral crystallization; enzymatic racemization
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| E-Mail |
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| 원문파일 |
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