| 학회 |
한국고분자학회 |
| 학술대회 |
2017년 가을 (10/11 ~ 10/13, 제주컨벤션센터) |
| 권호 |
42권 2호 |
| 발표분야 |
고분자구조 및 물성 |
| 제목 |
A highly efficient protein delivery system mediated by cell penetrating peptide pVEC into microalgae |
| 초록 |
Intracellular delivery of proteins is quite an important technique in biological science. Delivery of proteins into cells enables the investigation of protein-protein interactions and their functions in a cell system. Here, we propose a cell penetrating peptide pVEC-mediated protein delivery tool for microalgae. We discovered that the peptide pVEC from vascular endothelial cadherin was effective in transporting exogenous proteins into algal cells. This method was shown to deliver proteins of various sizes from 6 kDa to 150 kDa into wild-type Chlamydomonas and to also work in other algal species. In addition, we show that our proposed pVEC-mediated protein delivery system is based on both the endocytic and non-endocytic pathways simultaneously through a mechanism study using inhibitors. The results show that the proposed simple and efficient protein delivery tool could contribute to advanced algal research for a wide array of applications for microalgae in various industries. |
| 저자 |
강성수, 김유천
|
| 소속 |
카이스트 |
| 키워드 |
cell penetrating peptide; protein delivery
|
| E-Mail |
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