| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Antibiotic-free auxotrophic plasmid maintenance system for stable and tunable gene expression |
| 초록 |
Although benefits of multi-copy expression of the genes, plasmids require antibiotics despite of the concerns over economic and environmental problems. Here, we developed the antibiotic-free auxotrophic plasmid maintenance system for stable and tunable gene expression by precise control of plasmid copy number (PCN) in Escherichia coli. To achieve this, we expressed essential gene, infA encoding translation initiation factor (IF-1), on plasmid instead of chromosome. With this system, the gene expression was stably maintained for 40 generations with minimized cell-to-cell variation under antibiotics-free condition. Moreover, the PCN was rationally tunable in 5.6-fold by varying expression of infA. This antibiotic-free PCN control system enabled improvement on the production of both itaconic acid and lycopene (2-fold) compared to that of the conventional system. Collectively, the developed strategy could be a platform for production of value-added products in antibiotic-free cultivation. |
| 저자 |
강채원1, 정규열1, 임현규1, 양진아2, 노명현1, 서상우2
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| 소속 |
1포항공과대, 2서울대 |
| 키워드 |
Synthetic auxotrophy; Plasmid copy number; Expression control; Antibiotic-free; Stable expression; Plasmid stability
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| E-Mail |
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