| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Circuit-guided synthetic acclimation for improving biochemical production by microbial consortia |
| 초록 |
Although microbial consortia have been considered potential platforms for bioprocessing applications, the complexity in process control owing to the use of multiple strains necessitates the use of an efficient population control strategy. Herein, we report a novel population control strategy for improving biochemical production by a microbial consortium using circuit-guided synthetic acclimation. As a proof of concept, we designed a consortium comprising alginate-utilizing Vibrio sp. dhg and 3-hydroxypropionic acid (3-HP)-producing Escherichia coli strains for the direct conversion of alginate to 3-HP. We constructed a genetic circuit, named “Population guider”, which degrades ampicillin only when 3-HP is produced. We observed that 3-HP production by an acclimated population increased by 4.3-fold compared to that by a simple co-culturing consortium during a 48-h fermentation. We believe this concept is a useful strategy for the development of robust consortium-based bioprocesses. |
| 저자 |
강채원1, 정규열1, 임현규1, 원재혁2, 차상학1, 신기영1, 양재성1, 성재영2
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| 소속 |
1포항공과대, 2중앙대 |
| 키워드 |
Microbial consortia; Synthetic acclimation; Cooperation relationship
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| E-Mail |
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