| 학회 |
한국공업화학회 |
| 학술대회 |
2008년 봄 (05/09 ~ 05/10, 한양대학교(안산)) |
| 권호 |
12권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Production of Ortho-hydroxydaidzein Using Recombinant S. avermitilis Cytochrome P450 |
| 초록 |
Hydroxydaidzein shows significantly stronger antioxidative activities than those of daidzein. P450s have the ability of converting daidzein to hydroxydaidzein, 3΄-hydroxy, 6-hydroxy, and 8-hydroxydaidzein. We compared the various strains for the biosynthesis of dihydroxydaidzeins. Among strains examined, Streptomyces avermitilis MA4680 highly transformed daidzein to ortho-specific dihydroxyisoflavones. We investigated recombinant streptomyces avermitilis strain found to have 3.6 times higher ability than wild type to convert 3΄-hydroxydaidzein. The reaction conversion was maximized through fermentation. Fermentation result showed 4.4 fold increases in daidzein conversion activity compare to WT S. avermitilis. |
| 저자 |
이나훔, Bishnu Pandey, 노창현, 고성기, 최권영, 김태진, 홍은영, 김병기
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| 소속 |
서울대 |
| 키워드 |
Ortho-hydroxydaidzein |
| E-Mail |
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