| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 봄 (04/10 ~ 04/11, 대전 컨벤션센터) |
| 권호 |
39권 1호 |
| 발표분야 |
의료용 고분자 부문위원회(초청-영어발표/포스터-국문발표 가능) |
| 제목 |
Synthetic polymers for non-viral gene delivery system to human turbinate mesenchymal stem cells (hTMSCs) |
| 초록 |
Gene delivery using non-viral vectors has received a great deal of attention because of ease in preparation, stability, and safety. The aim of this study is to evaluate transfection efficiency hTMSCs using DNA/polyethyleneimine (PEI) complexes. DNA/PEI complexes with N/P charge ratios in the range 1–16 were formed by adding appropriate amounts of PEI to the DNA. The formation of complexes was characterized by gel electrophoresis, measurement of particle size and zeta potential. The viability of hTMSCs was conducted using the MTT assay. After gene transfection, green fluorescence was monitored by microscope and FACscan. DNA was completely condensed with PEI above N/P 1-2. As the portion of PEI was increased, the particle size of complexes decreased, the surface charge and cytotoxicity to hTMSCs were increased. The transfection efficiency was 47% at N/P 8 in 24 hours. Our study demonstrates that PEI may be applied as an effective gene carrier to hTMSCs. |
| 저자 |
권진선1, 박승훈1, 장자용1, 백지혜1, 김성원2, 김문석1
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| 소속 |
1아주대, 2카톨릭대 |
| 키워드 |
polyethyleneimine; gene delivery; stem cell
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| E-Mail |
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