| 초록 |
When we have a disease, there are so many therapy methods we can use such as injection or surgery. One of them, treatment using stem cell is very basic and important method. In this work, we purpose human turbinate mesenchymal stromal cells (hTMSCs) to differentiate to osteoblast using methoxy polyethylene glycol-polycaprolactone block copolymer (MPEG-PCL) and osteogenic factor. First, we synthesized MPEG-PCL hydrogel and measured viscosity. In order to demonstrate osteogenic ability of hTMSCs, we conducted in vitro osteogenesis experiment. Then, to confirm the cell cytotoxicity, we performed WST-1 with hTMSCs and MPEG-PCL. As the result of in vitro experiment, we implanted cell and hydrogel mixture in to animal model and checked degree of osteogenesis with histological analysis and amount of expression genes. Through these experimental data, we are sure that hTMSCs are very good source of osteogenesis with hydrogel and will use it to tissue engineering as important treatment method. |
