| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 봄 (04/09 ~ 04/10, 대전컨벤션센터) |
| 권호 |
34권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Effect of chemical structure and Molecular Weight of PolyethylenimineGene Carrier on the Reverse Transfection-mediated Transgene Expression |
| 초록 |
Gene delivery by non-specific adsorption of non-viral vectors to protein-coated surfaces can reduce the amount of DNA required, and also increase transgene expression and the number of cells expressing the transgene. Nucleic acids can be complexed with polymer to form DNA nanoparticles which are then immobilized on the surface coating extracellular matrix protein (ECM), termed as reverse transfection. We investigate the effect of molecular weight (Mw) and 3-dimensional structure of positive-charged PEI on transfection efficiency via surface-mediated delivery by changing Mw and structures of PEI. Different types of PEI (branched PEI Mw 25kDa, linear PEI Mw 1.8kDa, linear PEI Mw 10kDa, and linear PEI Mw 25kDa) were examined for enhanced transfection to neighboring cells. Our initial results indicate that branched type displays higher gene transfer and that higher Mw produces better transfection to neighboring cells. |
| 저자 |
차혜련1, Chau Nguyen2, 이상준2, 정서연3, Hieu Quang4, 김용숙5, 권진숙5, 정명호5, 안영근5, 박인규4
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| 소속 |
1전남대병원 심장질환 치료기술 개발 특성화 센터 / 전남의대 의생명과학과, 2전남의대 의생명과학과 / 전남의대 임상백신연구개발사업단, 3전남의대 의생명과학과 / 광주과학기술원 바이오 광학 영상센터, 4전남의대 의생명과학과 / 전남대병원 심장질환 치료기술 개발 특성화 센터, 5전남대병원 심장질환 치료기술 개발 특성화 센터 |
| 키워드 |
gene delivery; reverse transfection; nanoparticles
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| E-Mail |
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