| 초록 |
Injectable alginate hydrogels offer a wide range of attractive benefits. In this study, enzyme-mediated injectable pullulan hydrogel containing blood coagulation agents such as thrombin and calcium chloride were fabricated to use as a hydrogel type hemostatic agent. The pullulan hydrogel has many advantages such as biodegradability, flexibility, and blood coagulability. The modified pullulan was analyzed by ATR-FTIR, 13C NMR and 1H NMR to confirm introduction of tyramines. Gelation, rheological properties were determined as a function of concentration of horseradish peroxidase and pullulan solutions. The in vivo animal study showed that the modified hydrogel significantly reduced hemorrhage quantity and bleeding time compared to the untreated model. As a result, the modified pullulan hydrogel containing blood coagulation agents had suitable gelation time, biodegradability and hemostatic ability and provided an effective hemostatic agent to control massive blood loss. |
