| 초록 |
Rationally designed micro-sized RNA particles, called RNA microspheres (RMSs), were generated with single or double strands via rolling circle transcription. The RMSs showed an exceptional stability in the presence of serum, while they were selectively degraded under endolysosomal conditions. With precisely controlled size, both RMSs were successfully taken up by macrophages. Unlike the nature of RNA fragments, RMSs induced only basal-level expression of inflammatory cytokines as well as type I interferon from macrophages, suggesting that RMSs are immunocompatible in the therapeutic dose range. This study could help accelerate clinical translation and broaden the applicability of the self-assembled RNA-based particles without being limited by their potential immunotoxicity, while a systematic controllability to release RNA fragments from RMSs would provide self-assembled RNA-based structures with a great potential for immunomodulation. |
