| 초록 |
Tuberculosis (TB) is the major global cause of death from a single infectious agent, Mycobacterium tuberculosis (Mtb). A long-time cultivation of Mtb is necessary to confirm Tuberculosis. Although massive efforts are given for developing point-of care (POC) sensors, they suffers from its low sensitivity. Herein, we report a study of detecting Mtb by FRET particulate system with trehalose analog, which is an essential metabolite in Mtb. Ag85, a major secretion protein, catalyses trehalose during cell wall synthesis. When incubated with Mtb, the FRET particles exhibit a change in FRET ratio due to release of dye following the rupture of particles. We optimized the particulate system for sensitivity and stability. In a pre-test, the detection system showed an increase in FRET ratio with a small amount of Ag85 protein. In subsequent experiments, the optimized particles will be incubated with mycobacterium directly. |
