| 초록 |
Enzymatically cross-linked hydrogels via a horseradish peroxidase (HRP)/H2O2-catalyzed reaction have recently received much attention in biomedical fields due to easy tunable hydrogel properties. However, initial high level of H2O2 can cause DNA damage and cell death, and lead to a decrease of HRP catalytic activity. In this study, we report novel in situ H2O2 generation strategy using glucose oxidase (GOx)-mediated reaction to minimize effect of excess H2O2 during HRP-catalyzed hydrogelation. This system showed easy control of hydrogel properties (gelation time; 10-600 s, mechanical strength; 2-20 kPa) by varying the GOx and glucose concentration. In vitro 3D cell study demonstrated relatively enhanced cell cytotoxicity in HRP/GOx-mediated system than in HRP/H2O2 system. In conclusion, novel in situ cross-linkable hydrogels formed by dual enzyme-triggered reaction have a great potential for tissue engineering applications. Acknowledgements: NRF-2015M3A9E2028577 and NRF-2009-0093826 |
