| 초록 |
Catechol has been widely introduced to polymeric biomaterials due to its surface-independent adhesiveness. As its molecular similarity with phenols, catechol may have the ability for peroxidase-mediated enzymatic cross-linking, which has been poorly exploered. In this study, dopamine (DA) conjugated Tetronic (TDA) hydrogel was prepared as an injectable and bioadhesive scaffold. Tetronic was conjugated with DA using a carbodiimide-based chemistry. TDA hydrogel was formed via enzymatic crosslinking by horseradish peroxidase and H2O2. The elastic modulus of TDA hydrogel was controllable in the range of 200−5000 Pa. The adhesive strength of TDA hydrogel showed six times higher than that of fibrin glue. Cyto-toxicity of TDA hydrogel was evaluated using fibroblast in the 3D environment and majority of cells were viable. Our results suggested that TDA hydrogel was formed successfully and has a potential as an injectable and bioadhesive matrix for biomedical applications. |
