| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.698 |
| 발표분야 | 분자전자 부문위원회 |
| 제목 | Surface Fabrication of Biotinylated polypeptide Multi-layer Designed for Surface Plasmon Resonance Sensing |
| 초록 | The novel modification of gold surfaces with a biotinylated polypeptide multilayer formed by nonspecific electrostatic adsorption was designed for SPR sensing platform. The multilayer of polypeptides (Poly-L-lysine and Poly(aspartic acid)) was prepared onto negatively charged self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) on gold film.1 To confirm the multilayers fabrication and evaluate a surface density, contact angle, zeta potential, AFM and XPS were investigated. The polypeptide multilayer were functionalized with biotin by directly grafting to poly(succinimide) (PSI).2 Avidin-biotin interactions as a typical protein-ligand model were investigated on this biotinylated polypeptide multilayer surface. The specific binding of avidin to the biotinylated polypeptide multilayer (DS 2.7%) resulted in a shift in the refractive index of 0.003 ± 9×10-4 with a surface density of 2.9 ng/mm2. This multilayer surface was found to provide a simple and effective method for the SPR sensing. Fig.1. The schematic diagram of a miniature, integrated SPR trasducer. Reference [1] Claire E. Jordan and Robert M. corn, Anal. Chem. 1997, 69, 1449 [2] J.H. Jeong, B.Y. Kim, S.H. Cho, and J.D. Kim, JNOPM, 2004 accepted |
| 저자 | 정재현1, 정보경1, 김병윤2, 조성호1, 김종득1 |
| 소속 | 1한국과학기술원, 2LG 화학 |
| 키워드 | SPR; Multilayer; polypeptide; biotinylated layer |
