| 초록 |
H2 production was compared by using the four different microorganisms. In batch fermentation by growing cells, effects of temperature and formate addition were studied and specific H2 production activity was measured by resting cells. Relatively higher temperature was required for H2 production and cell growth by E. coli K12 and E.coli DJT135 than C. ammalonaticus Y19 and E. aerogene. In the batch fermentation, genetic modified Escherichia coli DJT135 and E. aerogene showed a little higher H2 production yield (1.81~1.89 mol H2/mol glucose) than Citrobacter ammalonaticus Y19 and E.coli K12 (1.72 ~ 1.76 mol H2/mol glucose). Specific H2 production activity from formate under the various temperature and pH conditions by resting cells was measured. Maximum specific H2 production activity of Y19, K12, DJT135 and E. aerogene were 1.21, 0.70, 1.14 and 0.94 mmol H2/g cell•min, respectively. Relatively, E. coli DJT135 exhibited higher H2 production yield and specific activity than others. And Y19 showed the highest maximum specific H2 production activity even though a little low H2 yield. |
