| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-고분자 |
| 제목 |
Colorimetric multiplex immunoassay using hydrogel microparticles |
| 초록 |
Among the techniques used to detect for multiple proteins in solution, a bead-based immunoassay using hydrogel microparticle is one of the methods noted for its possibility to quantify multiple analytes with graphically encoded microparticle. However, it is still limited to practical use because protein-bound microparticles is labeled by fluorescent dye which has problem for fluorescence quenching and requires expensive equipment to analyse signal. Herein, we introduce colorimetric detection to bead-based assay using hydrogel microparticle. Using colour dyes generated in an amplifiable enzymatic reaction in the presence of a target proteins, which accumulate in pores of microparticle, particles are imaged and analysed using low-cost portable devices. |
| 저자 |
노윤호1, 이현지2, 김주연1, 김현웅1, 김선민3, 봉기완1
|
| 소속 |
1고려대, 2georgia tech, 3보라매병원 |
| 키워드 |
하이드로젤 입자; 유체리소그래피; 단백질 검출; 다중검출
|
| E-Mail |
|
