| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 봄 (04/09 ~ 04/10, 대전컨벤션센터) |
| 권호 |
34권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Proliferation and phenotype of Schwann cell and Olfactory ensheathing cell on Hyaluronic Acid/PLGA Scaffold by Emulsion Freeze-drying method |
| 초록 |
PLGA and hyaluronic acid(HA) has been widely used as biocompatible scaffolds materials to regenerate tissue. HA loaded PLGA scaffolds were prepared by a emulsion freeze-drying method. The SC and OEC were seeded on the HA-PLGA scaffolds and measured by MTT assay. Morphological observation (through SEM), histology, and PCR were performed. In MTT assay result, scaffolds containing HA were higher cell viability then only PLGA scaffolds. mRNA, s-100,GFAP, and NF were greater than HA-PLGA scaffolds. When we cultured SC and OEC of rat in vitro, we observed better to keep the characteristic of SC in the HA-PLGA scaffolds than that PLGA scaffolds. This study suggests that HA/PLGA scaffold may serve as a potential cell delivery vehicle and a structural basis for in vitro tissue engineered SC and OEC. Acknowledgements This research was supported by SCRC(SC4110) |
| 저자 |
정수현, 김혜린, 홍현혜, 김순희, 이동원, 유일수, 이종문, 강길선
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| 소속 |
전북대 |
| 키워드 |
PLGA; hyaluronic acid; SC; OEC; Scaffold
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| E-Mail |
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