| 학회 |
한국화학공학회 |
| 학술대회 |
2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 |
9권 1호, p.455 |
| 발표분야 |
생물화공 |
| 제목 |
Hepatitis C virus(HCV) polymerase detection using RNA aptamer with photoclevable linker in microfluidic chip |
| 초록 |
The detection and isolation of biomolecules by specific ligands can be performed on affinity chromatography. We used the RNA aptamer instead of generally used antibody as ligand for affinity chromatography in a microchip. Oligonucleotides, RNA or DNA are more stable than protein under room temperature and site specific modification is possible on beads since oligonucleotides can be modified by tailing reaction. And photolabile linkers sensitive to UV radiation was introduced with aptamers on beads so the bound target proteins was eluted by 330nm UV radiation. Through miniaturized microfluidic affinity chromatography (BAC) system using aptamers, rather mild elution condition was applied without harsh conditions which is normally used for conventional affinity chromatography. The HCV RNA replicase which captured by aptamer from serum was digested by trypsin and this peptides analyzed through MALDI-TOF .
|
| 저자 |
조수형1, 이상호2, 정우재3, 김용권2, 이윤식3, 김병기3
|
| 소속 |
1서울대, 2서울대 전기컴퓨터 공학부, 3서울대 응용화학부 |
| 키워드 |
Aptamer; microchip; photoclevable linker; Hepatitis C virus
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| E-Mail |
, |
| VOD |
VOD 보기 |
