| 초록 |
Hydrogen peroxide (H₂O₂) is one of the major reactive oxygen species (ROS), which play a critical role in a variety of normal physiological process and diseases mechanism. Due to its implication in disease pathology, in particular, oxidative stress in neurological disorders, developing methods for monitoring the generation of ROS has increasingly drawn attentions. Although a variety of H₂O₂ detection methods including enzymatic and electrochemical techniques have been developed, they have still some problems: poor sensitivity, reproducibility, selectivity, stability and in vivo applicability. Here, we report a novel H₂O₂ detection method based on single plasmonic nanoprobe conjugated with redox active Cytochrome c (Cyt c). We observed the dynamic spectral change in the fingerprint quenching dip of a single plasmonic nanoprobe in accordance with the redox state of the conjugated Cyt c induced by H₂O₂. Based on the spectral change of single plasmonic nanoprobe, we can detect H₂O₂ at physiologically relevant micromolar concentration range. We believe that our approach could pave the way toward dynamic, high spatial resolution monitoring of ROS in living cells in the future. |
