| 초록 |
ECM is a scaffolding material evolved by nature, not only to provide structural support to cells but also to regulate cell behaviors. In this study, we present porcine adipose tissue as a plentiful source of ECM. In particular, porcine adipose tissue is easily obtained from food waste. ECM was isolated and defatted by homogenization, and then decellularized through chemical and enzymatic treatments. After decellularization, immunogenic components such as DNA and α-Gal were significantly reduced, while abundant proteins were well preserved in the decellularized ECM. The biochemical cues of decellularized ECM could dramatically support the growth of human cells in vitro. Moreover, decellularized ECM exhibited good biocompatibility and long-term stability in vivo. Overall results suggest that the decellularized ECM could be useful as a xenogeneic biomaterial for xenograft tissue engineering. |
