| 초록 |
Exosomes are extracellular vesicles (EVs), which have attracted attention because of their potent a means of disease diagnosis and biocompatible carrier. However, because of difficulties in their capture and detection, further application of exosomes remains challenging. To detect EVs, we fabricated a liposomal biosensor based on polydiacetylene (PDA), a conjugated polymer that has been widely used in sensing applications because of its unique optical properties. To confer selectivity and sensitivity to the sensory material, anti CD-9 antibodies targeting CD-9, a membrane protein exclusively found in exosomes, were attached to the PDA liposome composites. To obtain PDA Signal triggered by the ligand-receptor interaction of PDA vesicles, Visual, UV-visible, and fluorescence spectroscopic methods were used, which achieved a detection limit of 3×108 vesicles/mL, the minimum concentration that can be used in practical applications. |
