| 초록 |
Klebsiella oxytoca naturally produces a large amount of 2,3-butanediol (2,3-BD), especially (2R,3S)-BD, a promising chemical for cosmetics. In this study, the in silico gene knockout simulation based on genome-scale metabolic model of K. oxytoca was carried out. Genomic analysis of K. oxytoca metabolism targeted the knockouts of ldhA and pflB genes. The constructed K. oxytoca ΔldhA ΔpflB strain showed higher (2R,3S)-BD yields and higher final concentrations than those obtained from the wild-type and ΔldhA strains. The simulation results showed that the agitation speed strongly affected cell growth and 2,3-BD synthesis. Then, we optimized the condition of fed-batch fermentation and produced 113 g/L of 2R,3S rich-BD, with a yield of 0.45 g/g, and for the production of 2.1 g/L/h of 2R,3S rich-BD. [This work was supported by the Biochemical Industry Promoting Technology Development Project (No. 10050407) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).] |
