| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 가을 (10/19 ~ 10/21, 대전컨벤션센터) |
| 권호 |
22권 2호, p.1791 |
| 발표분야 |
생물화공 |
| 제목 |
Ag@silica-Entrapped Hydrogel Microarray: A New System For A Metal Enhanced Fluorescence-Based Protein Assay |
| 초록 |
Recently, the fluorescence detection method utilizing metal-enhanced fluorescence (MEF) has been widely studied in an effort to improve the detection sensitivity of protein-based bioassays. MEF is now a well-established technology, wherein the interactions of fluorophores with metallic nanoparticles results in fluorescence enhancement. In this study, as one solution for the problems associated with plate- and particle-based platforms, we developed a novel silver-based MEF biosensing platform that consisted of poly (ethylene glycol)(PEG) hydrogel microstructures entrapping silica-coated AgNPs (Ag@silica). Hydrogels are three-dimensional polymeric structures that absorb water or other biological fluids, and as it have a soft and hydrated nature, biomolecules is retained their activation. So, We took advantage of the MEF from Ag@SiO2 within the hydrogel microstructures to improve the performance of the fluorescence detection device. |
| 저자 |
김민수, 한상원, 조강희, 방혜정, 민지홍, 고원건
|
| 소속 |
연세대 |
| 키워드 |
바이오칩/나노바이오공학 |
| E-Mail |
|
| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
