| 학회 | 한국화학공학회 |
| 학술대회 | 2016년 가을 (10/19 ~ 10/21, 대전컨벤션센터) |
| 권호 | 22권 2호, p.1793 |
| 발표분야 | 생물화공 |
| 제목 | Development of biocatalysts based on carbonic anhydrase for CO2 sequestration |
| 초록 | Carbonic anhydrase (CA) is an enzyme that catalyzes reversible hydration of CO2. It has been recently suggested that this remarkably fast enzyme can be used for sequestration of CO2, making this a promising alternative for chemical CO2 capture. For its practical application, we developed efficient and economic biocatalysts with high stability, mainly based on a recombinant CA originated from Neisseria gonorrhoeae (ngCA). First, we engineered ngCA in the periplasm of E. coli to promote the economical use of enzymes, thereby creating a bacterial whole-cell catalyst. Second, we developed and characterized bioinspired silica nanoparticle with recombinant ngCA autoencapsulated by the silica-condensing R5 peptide fused to the ngCA. Next, we designed and engineered de novo disulfide bond in ngCA in order to improve the thermostability of the enzyme. Finally, we found and characterized thermophilic α-CAs originated from Persephonella marina and Thermovibrio ammonificans, demonstrating their remarkable thermostabilities. |
| 저자 | 조병훈 |
| 소속 | 국립 경상대 |
| 키워드 | 미생물공학; 효소/단백질공학 |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
