| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
MR Imageable p53 Gene Therapy with PEI-tethered SPION |
| 초록 |
Targeting therapeutic drug using Superparamagnetic iron oxide nanoparticles (SPION) as carrier systems gained increased attention in the biomedical field. According to their desired purpose, they were also additionally functionalized with drugs, plasmids, peptides or proteins of interest. The p53 gene is a tumor suppressor and genome defense. Therefore, recovering the normal function in cancer cells by replacement of the aberrant p53 gene, the normal one is one of the most efficient and critical approaches for tumor gene therapy. In this study, we proposed gene carrying SPION for anti-cancer gene therapy. For the work, delivery of p53 tumor suppressor gene by bPEI-SPION obtained the suppressive effect on CT26, 4T1, Ca46 and Daudi. And internalized SPIONs were tracked by MR imaging. MR contrasts were strong and became more intense with increasing amounts of the bPEI-SPION. Protein expressions of p53 gene after transfection into the cell lines were measured by real-time and RT-PCR. |
| 저자 |
이화정1, 이상준1, 차혜련1, Hieu1, 문명주2, 정용연1, 이일권2, 박인규1, 남궁란3, 김원종3, 전상용4, 유미경4
|
| 소속 |
1전남대, 2화순전남대, 3포항공과대, 4GIST |
| 키워드 |
SPION; P53; Gene Therapy; MRI
|
| E-Mail |
|
