| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
SPION-encapsulated polymersome-mediated gene delivery guided by enhanced Magnetic resonance(MR) signal |
| 초록 |
Development of magnetic materials for diagnosis has been tried for several decades to identify the disease-related tissues. Recently, combination of SPION-based diagnostic properties with accompanying therapeutics has been explored to improve their therapeutic efficacy. In this regard, positively charged SPION-loaded polymersome tethering PEG as a hydrophilic corona layer was prepared to deliver therapeutic genes to target sites, which was monitored by MRI. The complex formation with plasmid DNA was investigated by gel electrophoresis. Particle size and morphology were measured by DLS and TEM, respectively. Hydrodynamic particle size of SPION-embedded complex was found to range around 200 nm, and its surface charge was positive determined by zeta potential measurement. The transfection efficiency in vitro was tested with luciferase-expressing plasmids/SPION-embedded complex. This result indicates that the SPION-embedded complex can be applied to MR imaging guided gene therapy. |
| 저자 |
이상준1, 이현진2, 문명주1, Hieu1, 이화정1, 차혜련1, 정용연1, 박인규1
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| 소속 |
1전남대, 2한국과학기술원 생명화학공학과 |
| 키워드 |
SPION; gene delivery; MRI
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| E-Mail |
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