| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Synthesis of Cell Penetrating Gene Carrier for Breast Cancer-targeted Therapy |
| 초록 |
A new drug delivery system with cell-penetrating pepetide (CPP) gained greater attention in biomedical filed. CPP facilitates intracellular uptake of target materials such as protein, gene, or macromolecules. Development of therapeutic molecule was greatly thwarted by poor uptake efficiency and low cellular trafficking, which might be overcome by chemical conjugation with CPP. In this study, we conjugated newly identified cellular penetrating peptide, which was found to be highly specific to breast cancer, to cationic gene carrier for achieving enhanced intracellular uptake. Tumor suppressor gene, p53, was delivered with CPP-conjugated gene carrier to breast cancer and its therapeutic efficacy was evaluated. The complex formation with plasmid DNA was performed by gel electrophoresis. Particle size and the surface charge were determined by DLS and zeta potential. The breast cancer transfection and targeting were tested with different breast cancer cell types and xenograft animal model. |
| 저자 |
이화정1, 문명주2, 정서연1, 남궁란3, 김원종3, 김재일4, 박인규1
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| 소속 |
1전남대, 2화순전남대, 3포항공대 화학과, 4GIST 생명과학과 |
| 키워드 |
Breast Cancer; Gene; Peptide
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| E-Mail |
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