| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Precise tuning of glyoxylate cycle for efficient conversion of acetate into value-added chemicals |
| 초록 |
Utilization of abundant and cheap carbon sources can effectively reduce the production cost and enhance the economic feasibility. Acetate is a promising carbon source to achieve cost-effective microbial processes. In this study, we engineered an Escherichia coli strain to produce itaconic acid and tyrosine from acetate. As acetate is known to inhibit cell growth, we initially screened for a strain with a high tolerance to 10 g/L of acetate in the medium. Thereafter, acetate uptake pathway and checmical production pathway were amplified. To further enhance the production, the activity of the glyoxylate cycle was precisely controlled by expression of isocitrate lyase gene under different-strength promoters. As the results, production of itaconic acid and tyrosine could be significantly enhanced and these efforts support that acetate can be a potential feedstock for biochemical production with engineered E. coli. |
| 저자 |
노명현1, 임현규1, 조민지1, 우성화1, 강채원1, 임대균2, 오민규2, 정규열1
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| 소속 |
1POSTECH, 2고려대 |
| 키워드 |
Glyoxylate cycle; Acetate; Tyrosine; Itaconic acid; Metabolic Engineering; Synthetic Biology
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| E-Mail |
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