| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Efficient Gene Delivery Systems Using Inverted Quasi-spherical (iQS) Culture Substrates for Cell-gene Therapy |
| 초록 |
In the field of cell and gene therapy, the genetically modified therapeutic cells have been studying for efficient medical approaches using viral or non-viral gene delivery vehicles. Using these approaches, the efficiency of gene delivery and expression levels are crucial for increasing therapeutic effects. Herein, the inverted quasi-spherical (iQS) system, which were fabricated with the hydrophilic dot patterns on the TiO2 coated superhydrophobic substrates, provided the steep curvature of hanging droplets during three-dimensional (3D) cell cultures. This unique microenvironment enhanced the cell-cell and cell-gene interactions. In this study, the human neural stem cells (hNSCs) were showed the enhanced transduction efficiency of adeno-associated viral vectors (AAV) and increasing of protein secretion levels of therapeutic cytokine using this iQS culture systems. |
| 저자 |
김승현1, 이미현2, 조미라1, 김일선1, 박국인1, 이해신2, 장재형1
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| 소속 |
1연세대, 2카이스트 |
| 키워드 |
3D culture; AAV; cell-gene therapy
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| E-Mail |
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