| 초록 |
In this work, we prepared a three-dimensional (3D) printed polycaprolactone scaffold (PCLS) grafted with recombinant human bone morphogenic protein-2 (rhBMP2) attached via polydopamine (DOPA) chemistry. The developed scaffolds were physico-chemical analyzed. For the In-vitro cell study, pre-osteoblast MC3T3-E1 cells were seeded onto PCL scaffolds (PCLS), DOPA coated PCL scaffold (PCLSD), and scaffolds with varying concentrations of rhBMP2 grafted. These scaffolds were evaluated by cell test. Through these studies, we demonstrated that our fabricated scaffolds were well coated with DOPA as well as grafted with. This grafting enables rhBMP2 to be released in a sustained pattern. In the in-vitro results, the cell proliferation and an osteoconductivity of PCLSD 500 groups was greater than any other group. All of these results suggest that our manufactured 3D printed porous scaffold would be a useful construct for application to the bone tissue engineering field. |
