| 학회 |
한국공업화학회 |
| 학술대회 |
2012년 봄 (05/09 ~ 05/11, 김대중컨벤션센터) |
| 권호 |
16권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Preparation of PhotoreactiveAzidophenyl Hyaluronic Acid Derivative: Protein Immobilization for Medical Applications |
| 초록 |
Photoreactiveazidophenyl hyaluronic acid (Az-HA) derivative was synthesized to immobilize proteins such as growth factor. The photosensitizing group, 4-azidoaniline, was introduced to the carboxyl group of hyaluronic acid by a water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC). To characterize the synthesized Az-HA, FTIR,1H NMR, and UV/VIS absorbance spectroscopy were performed. We demonstrated protein immobilization by using fluorescein isothiocyanate conjugated albumin (FITC-BSA) on Az-HA derivative surfacesthatwere patterned with micro scale. Cytotoxicity and relative cell proliferation tests of the Az-HA derivative were performed on 3T3-L1 fibroblast cell lines for biocompatibility. |
| 저자 |
이형재1, 박신혜1, 서시웅1, 조영민1, 허윤1, 우희동1, Yoshihiro Ito2, 손태일1
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| 소속 |
1중앙대, 2RIKEN Advanced Science Institute |
| 키워드 |
Hyaluronic acid; 4-azidoaniline; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; Photoreactive; Protein immobilization
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| E-Mail |
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