| 학회 |
한국공업화학회 |
| 학술대회 |
2005년 봄 (05/13 ~ 05/14, 충남대학교) |
| 권호 |
9권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Soluble expression of aggregation-prone proteins in a cell-free protein synthesis system |
| 초록 |
As the demand for a faster and more parallel expression system becomes greater in the post-genomic era, cell-free protein synthesis is now actively considered as an alternative method for high-throughput gene expression. In contrast to in vivo gene expression, cell-free protein synthesis provides a completely open system that allows direct manipulation of the environment for transcription and translation. The flexibility of cell-free synthesis is demonstrated with our results showing the soluble expression of aggregation-prone proteins through simple modification of reaction conditions. We present our recent results indicating that the solubility of cell-free expressed proteins can be substantially enhanced through the additions of molecular chaperones and/or chemical detergents into the synthesis reactions. |
| 저자 |
박창길1, 권용찬1, 이경호1, 한근희1, 안진호2, 김태완2, 최차용2, 김동명1
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| 소속 |
1충남대, 2서울대 |
| 키워드 |
cell-free protein synthesis; molecular chaperones; chemical detergents
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| E-Mail |
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