| 초록 |
Diacetylene lipid monomers could form vesicles and polydiacetylenes(PDA) could be made by photopolymerization of self-assembled diacetylene monomers. Among the various diacetylene lipids, 10,12-pentacosadiynoic acid (PCDA) is one of the most extensively studied target materials for the preparation of various polydiacetylene structures and the platform application technology of detecting biomolecules as well as diverse environmental stimuli. Visible color change from blue to red occurs in response to a variety of environmental perturbations. Many studies have been reported about using cationic polymer for non-viral vectors. In this study, we introduced cationic amino acids at the surface of PCDA liposome using SPPS ( solid phase peptide synthesis ) methods and prepared liposome solutions at various molar ratios. When the liposomes were transfected at several mammalian cells, high transfection efficiency and low toxicity was observed. |
