| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Development of Biodisc Tissue-Engineered Using Annulus Fibrosus and Nucleus Pulposus Cell on PLGA/DBP Scaffold |
| 초록 |
We fabricated poly(lactide-co-glycolide)(PLGA) scaffolds impregnated demieneralized bone particle(DBP)(PLGA/DBP)content to investigate the effect of cell viability and proliferation of intervertebral disc(IVD) cells. Annulus fibrosus(AF) and nucleus pulposus(NP) cells were seeded in PLGA and PLGA/DBP scaffold to evaluate mRNA expression and a proliferation of AF and NP cells according to content of DBP. Reverse transcription polymerase chain reaction(RT-PCR) was assessed to measure of mRNA expression. Scaffolds were implanted in subcutaneous of athymic nude mouse for histology in vivo. The formation of disc-like tissue appeared in PLGA/DBP scaffold than control. Especially, 40 and 80 wt% of DBP positively affected to proliferation and maintenance of phenotype in scaffold. These information would contribute to design of PLGA/DBP scaffold for tissue engineered IVD regeneration. Acknowlegement: This research was supported by KMOHW (0405-BO01-0204-0006) and SCRC (SC3100). |
| 저자 |
임지예1, 김순희1, 소정원1, 백미옥1, 홍현혜1, 정수현1, 최진희1, 이종문1, 이해방2, 강길선1
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| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
PLGA; DBP
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| E-Mail |
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