| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 봄 (04/25 ~ 04/27, 창원컨벤션센터) |
| 권호 |
24권 1호, p.337 |
| 발표분야 |
생물화공 |
| 제목 |
Designed Immobilization of Carbonic Anhydrase for Efficient Enzymatic CO2 Sequestration |
| 초록 |
Carbonic anhydrase (CA) is a prominent biocatalyst used for the enzymatic CO2 capture process. For industrial applications, the immobilization of enzyme on a matrix support is a useful technique for stabilizing the enzyme and improving its reusability. In this study, we investigated the effect of the immobilization position on the stability of CA using α-type Bovine CA (bCAII). Several CA mutants with a single mutation to Cys in a different position were designed for site-specific immobilization on support materials. Enzyme stability is typically related with the structural flexibility of the enzyme. The enzyme structure can be divided into several substructures according to the distinctive flexible features of each domain, and the flexibility of each part of the enzyme could contribute to the structural stability. We decopmosed the structure of bCAII into six substructures via a quasi-rigid domain analysis, and one candidate residue was selected considering the exposure ratio based on solvent accessibility. The selected residues were mutated to Cys for site-specific immobilization, and their effects on the thermostability and long-term stability of the CA were investigated. |
| 저자 |
백승필, 손려강, 박기성, 김성호, 김종기, 여기백, 민기하, Thi Khoa My Nguyen
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| 소속 |
고려대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
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