| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Removal of Ni2+ and Cd2+ by Surface Display of Polyhistidine on Bacillus subtilis Spore using CotE Anchor Protein |
| 초록 |
We studied removing heavy metal using Bacillus subtilis spore surface display system. We used cot E protein as an anchoring motif because of its high abundance in coat layer and inserted varying length of histidine tag at the C-terminal end of anchoring motif. We checked the surface expression of histidine tag using flow cytometry with FITC labelled anti-his6-antibody. Histogram of flow cytometry showed higher fluorescence intensity, possibly due to their outer location on Bacillus subtilis spore. We tried heavy metal adsorption with three kind of recombinant spore and DB104(wild type). |
| 저자 |
김준형, 김우일, 백상근, 조영기
|
| 소속 |
Dong-A Univ. |
| 키워드 |
Bacillus subtills; CotE; Heavy metal
|
| E-Mail |
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