| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 |
14권 1호, p.436 |
| 발표분야 |
생물화공 |
| 제목 |
Proteomic and transcriptomic analysis for molecular mechanism in capsaicin-induced apoptosis between human hepatocarcinoma and human neuroblastoma cells |
| 초록 |
It was found that endogenous ROS levels were increased during hepatocarcinoma (HepG2) apoptosis, whereas they decreased during neuroblastoma (SK-N-SH) apoptosis in response to capsaicin treatments. To clarify this different phenomenon, we used 2-DE-based proteomics to analyze the altered protein levels in both cells, with special attention on oxidative stress proteins before and after capsaicin treatments. The 2-DE analysis demonstrated that 23 proteins were increased and 26 proteins were decreased in capsaicin-treated apoptotic HepG2 and SK-N-SH cells, respectively. Next, the global gene expression for oxidative stress and antioxidant defense genes was analyzed by the Human Oxidative Stress and Antioxidant Defense RT² Profiler™ PCR Array. Surprisingly, the profiles of 84 gene expressions related to oxidative stress and antioxidant defense were not significantly different in HepG2 cells between control and capsaicin-treated cells. In contrast, a number of oxidative genes were down-regulated in SK-N-SH cells, supporting the evidence of low ROS environment in apoptotic SK-N-SH cells after capsaicin treatment. |
| 저자 |
김현아1, 백유미2, 황희선1, 이성학2, 윤종원1
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| 소속 |
1Department of Biotechnology, 2Daegu Univ. |
| 키워드 |
Proteomic; capsaicin; hepatocarcinoma; neuroblastoma
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| E-Mail |
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| 원문파일 |
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