초록 |
Corynebacterium glutamicum, a lysine fermentation industry waste, showed promise for the removal of Reactive black 5 (RB5) from aqueous solution. Two polymeric materials, calcium alginate and polysulfone, were evaluated as possible immobilization matrices for protonated biomass of C. glutamicum. Optimization experiments revealed that 4% (with bead dosage of 2 g/40 ml) and 14% (with bead dosage of 1 g/40 ml) in the case of alginate and polysulfone beads, respectively, were identified as the favorable condition for RB5 biosorption. According to the Langmuir model, maximum RB5 uptakes of 351.9, 282.1 and 290.7 mg/g were observed for free, alginate and polysulfone immobilized biomass, respectively. In an effort to enhance process economy, biomass was regenerated and reused for number of cycles. Sorption-desorption experiments carried out for twenty consecutive cycles revealed that polysulfone beads exhibited invariable RB5 uptake capacity and very high mechanical stability (no significant weight loss) even at the end of twentieth cycle. |