| 초록 |
The number of alopecia increase constantly, and the rate of younger patient are growing. Using herbs to treat hair loss, a lot of research has been ongoing. There are few report the effect of Urtica Canabina on hair follicle. Recently, several in vitro assay systems have been reported as a means of investigating the mechanism of hair growth control. The objectives of this study were Urtica Canabina derived Mongolian effect proliferation and activity of human dermal papilla cell. The leaves of Urtica Canabina were extracted with hexane, methanol, and hot water. The extract was then concentrated in a vacuum evaporator. We seed 4 x 104 human hair derived cells each of wells in the 12 well plate. The day after, we added the diluted Urtica Canabina solution on cell culture media and cultured h-DPCs or h-ORSCs for 4 days. We performed LDH, MTT, RT-PCR, and topical test with C57BL6 mouse. Urtica Canabina extract did not stimulate h-DPCs proliferation but had some positive effect hair cell activity. |
