| 학회 | 한국화학공학회 |
| 학술대회 | 2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 | 10권 1호, p.209 |
| 발표분야 | 분리기술 |
| 제목 | Solid-Phase Refolding Technologies in Biopharmaceutical Protein Processing |
| 초록 | We have developed a several interesting process based on the solid-phase technology. “Solid-phase refolding” of inclusion body proteins uses ion exchange resins to adsorb denaturant-dissolved inclusion body. As the denaturant is slowly removed, the protein refolds into a native structure. The refolded protein can be eluted by a conventional elution technique. This concept was extended to “EBA-mediated refolding,” in which the denaturant-dissolved inclusion body in a whole cell homogenate is adsorbed to a STREAMLINE matrix as cell debris are removed by the EBA (expanded bed adsorption) action. The adsorbed protein follows the same refolding steps. This process shows the potential to improve the refolding yield, reduce the number of processing steps and the processing volume and time, and thus improve the overall process economics significantly. In this presentation, the background and the processing details involved in these applications will be discussed. |
| 저자 | 서창우, 김민영, 이은규 |
| 소속 | 한양대 |
| 키워드 | solid-phase refolding; inclusion body; expanded bed adsorption chromatography |
| 원문파일 | 초록 보기 |
